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KMID : 0379319910160010079
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Korean Journal of Rural Medicine
1991 Volume.16 No. 1 p.79 ~ p.89
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Studies on the Applicability of Various Antigen Preparations in the Immunoblot Diagnosis of Cysticercosis
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高永泰/Koh, Young-Tae
朱炅煥/鄭明淑/林漢鐘/Joo, Kyoung-Hwan/Chung, Myung-Sook/Rim, Han-Jong
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Abstract
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A systematic study was conducted to identify and isolate a serologically pertinent antigen with high specific activity and low cross reactivity from Cysticercus parenchymal antigen.
Differential centrifugation of the homogenate yield three particulate and one soluble fractions ; the 48OXG pellets(CyL2), the 7650XG pellet(CyL3), the 100000XG pellet(CyL4), and 100000XG supernatant(CyL6). We compared antigenicity of these antigens to that of cystic fluid antigens(CyF1), saline extract of cystic wall(CyL1), and n-butanol treated CyL4 antigen (CyL5) based on SDS-PAGE and immunoblot techniques.
The data obtained were as follows :
1) The ratio of O.D. value of ELISA against cysticercosis positive pool sera to that of negative pool sera was highest when using CyF1 as antigen. However, the ratio was relatively low in case of CyL3.4 and CyL5.
2) We have noted in previous paper that most strong antigenic activities are present in 63Kd band with low cross reactivities. An effective serologic reagent must contain components that are recognized by most of infected sera. 63Kd band met this criteria and could be considered as a reliable band for the diagnosis of cysticercosis. As far as 63Kd band concern. CyL5 showed most strong activities without disturbance of cross reaction by Eli B in spite of low applicability to microplate ELISA.
3) CyL5 could detect the serum antibody of cysticercosis even in very low titers, around cut-off values of microplate ELISA, by immunoblot It also could detect the cross reactivates of Echinococcus species which showed high absorbance value in micro plate ELISA and some sparganosis cases. Further purification of this antigen will be able to represents a antigen that can be used in the diagnosis of cysticercosis.
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